Preparative High Performance Liquid Chromatography (HPLC) for Biopharmaceuticals: Hipersep® Bio Line

 

Novasep has more than 20 years experience in polishing fragile molecules such as insulin, interferon, EPO, factor VIII.

 

 

Novasep Process has designed, built and sold the largest number of industrial HPLC systems for protein polishing in the world.

 

Typical Applications

Polishing of:

Insulin Interferon EPO HPLC Prochrom® Columns (See our Prochrom® DAC Columns for HPLC)

Factor VIII Lipids Polysaccharides

   

Hipersep® Bio - Product Line

High Performance Gradient

The mass-flowrate control and the in-line mixing system ensure highest precision (± 0 .1%) of composition of isocratic step and gradients.

Smart Fraction Collection System

the system is based on:

• time and/or detection threshold and/or slope
• volume
• time/volume after an event

Fully Customized Systems

Novasep Process engineers design customized units, ideally suited to your facilities and specific process needs.

Hipersep®	LAB I	LAB II	S	M	L	XL
Productivity range (kg/day)	0.01 - 0.2	0.02 - 0.3	0.1 - 5	0.2 - 20	0.5 - 40	40 - 140
Typical Prochrom® column diameters (mm)	50 80	80 110	150 200	200 450	300 600	600 1200
Flow rate (L/h)	6 - 36	12 - 54	60 - 150	150 - 1000	300 - 2000	500 - 2500
Standard max operating pressure (bar)	70	70	70	70	70	70
Number of fractions * optional	5 to 10 *	5 to 10 *	5 to 10 *	5 to 10 *	5 to 10 *	5 to 10 *
Dimensions W x H x D (cm)	88 x 123 x 70	88 x 123 x 70	195 x 175 x 100	225 x 195 x 110	225 x 205 x 160	260 x 205 x 170
Average weight (kg)	300	350	675	1250	1475	2500

Software - Hipersep® SE-Bio

• PLC controlled with PC user interface
• multiple safety levels
• user-friendly, designed for chromatographers and producers
• validated software compliant with 21 CFR part 11 and GAMP 4

Options

• injection pump
• backflush, recycling
• temperature control
• additional detectors: pH, conductivity, IR (standard UV detectors)
• design for ATEX zone
• automated wiped or falling film evaporators
• tankfarms and solvent recovery systems
• others

Novasep Process Biopharma PPD Services

Novasep Process offers complete process development services , and provides performance guarantees for processes supplied.

Benefits and Advantages

Experience

A long track record thanks to more than 20 years experience in polishing insulin, interferon, EPO, factor VIII.

Novasep Process has designed, built and installed the largest number of industrial HPLC systems for protein polishing.

Scalability

HPLC systems incorporate columns from a few millimetres up to 1.6 m internal diameter. Modeling tools allow easy scale-up with similar performance and optimized fluid dynamics.

Expertise

Unique teams of chemists, biochemists, process and computer engineers combine their complementary talents to develop and continuously improve purification processes.

Other

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