Low and Medium Pressure Chromatography (LPLC & MPLC): Hipersep® Bio line

For more than 10 years, Novasep Process has been designing, building and selling optimized Prochrom® biochromatography columns and systems for your biopharma purification applications.

Typical Applications

Capture and purification of

• Proteins, e.g.:
  • Monoclonal antibodies
  • Blood/plasma factors
  • Recombinant proteins
• Peptides
• Polysaccharides
• Oligonucleotides
• Viruses

Using, for example, affinity, ion exchange, hydrophobic interaction chromatography media.

Prochrom® Columns - Product Line

Standard Model Catalog Numbers

Low Pressure	Medium Pressure	Max Bed Volume (L)
LP.200.500XX5	MP.200.500 VE20	10
LP.300.500XX5	MP.300.500 VE20	25
LP.450.500XX5	MP.450.500 VE20	50
LP.600.500XX5	MP.600.500 VE20	100
LP.800.500XX5	MP.800.500 VE20	180
LP.1000.500XX5	MP.1000.500 VE20	270
LP.1200.500XX5	MP.1200.500 VE20	400

XX for either :
- VE: Dynamic Axial Compression
- MO: Manually Operated Piston

-simple
-column up to 80 cm i.d.

Features & Benefits of Prochrom® Biochromatography Columns

 

Features	Benefits
Pack & unpack in place features	Fast and easy column packing and unpacking in a closed contained system, maintaining optimal conditions for flow distribution
Pressure rating: low pressure columns (bar): up to 5 medium pressure columns (bar): from 5 to 20	Application of a wide range of bioprocessing media, high flow rates
Column diameter (mm): from 200 to 2000	Scale-up guaranteed
Bed height (mm): from 20 to 500	Flexibility for various applications
Jack at the top or at the bottom	Flexible design depending on customer and process requirements
Uniform flow distribution devices	High separation performance
Materials of construction: 316 L stainless steel, acrylic, glass: borosilicate type I, hastelloy, Teflon® seal (compatible with all organic solvents) or EPDM - full material certification package	High quality materials, which allow for a wide range of applications, compatible with all common process solutions
Safety & ergonomic tools	Easy to operate and maintain
Sanitary fittings, internal finish better than 0.4 µm, electropolished	Sanitary design, "clean solution"

  

Features & Benefits of Low and Medium Pressure Hipersep® Bio Liquid Handling Systems

Novasep Process engineers can design customized units incorporating columns up to 2000 mm internal diameter, ideally suited to your facilities and specific process needs.

Hipersep® Bio Liquid Handing Systems can handle flexible number of buffers & CIP inlets and up to 10 fractions.

Hipersep® Bio	S	M	L	XL
Flow rate (L/h)	15 - 150	50 - 500	150 - 1500	300 - 3000

Smart Fraction Collection System

• based on time and/or detection threshold and/or slope
• based on volume
• based on time/volume after an event

Quality

• complete IQ/OQ and PQ (PQ optional)
• ASME-BPE: code of construction

Options

For Prochrom® Columns:

• Dynamic Axial Compression (DAC): performance stability guaranteed, reproducible results, simplicity of use, adjustable bed height
• manual operated piston
• patented Intelligent Piston: protects from over pressurization of packed beds
• jacket: temperature controlled processes, no need to process in cold rooms

For Hipersep® Bio:

• temperature control
• design for ATEX zone
• tankfarms and solvent recovery systems
• column packing units
• SIP
• others

Software - Hipersep® SE-Bio

The Hipersep® SE-Bio software operates and monitors all LPLC and MPLC systems according to customers' needs:

• isocratic, step or linear gradients
• in-line buffer preparation
• design for bio-applications
• validated software compliant with 21 CFR part 11 and GAMP 4
• user-friendly, designed for chromatographers and producers

Novasep Process Biopharma PPD Services

Novasep Process offers complete process development services, and provides performance guarantees for processes supplied.

Benefits and Advantages

Experience

For more than 10 years, Novasep Process has been designing, building and selling optimized Prochrom® biochromatography columns and systems for your biopharma purification applications.

Novasep Process provides product and process development and fully-automated chromatographic systems that integrate easily and perfectly in your process.

Scalability

LPLC and MPLC systems incorporate Prochrom® biochromatography columns with bed volumes from 10 to 400 L and column diameter from 200 mm to 2000 mm. We can develop custom-made design on request.

We provide Prochrom® biochromatography columns for your scale-up solution.

Expertise

Unique teams of chemists, biochemists, process and computer engineers combine their complementary talents to develop and continuously improve therapeutic products capture and purification.

Other

Please challenge us!