Preparative Low and Medium Pressure Liquid Chromatography (LPLC, MPLC): Hipersep®-Bio

For more than 20 years, Novasep has been designing, building and selling optimized biochromatography systems for your biopharma purification applications.

Typical Applications

Capture and purification of:

• Therapeutic proteins: e.g.:

• Monoclonal antibodies (mAbs)
• Blood/plasma factors
• Recombinant proteins
• Peptides
• Polysaccharides
• Oligonucleotides
• Viruses

Using, for example, affinity, ion-exchange, hydrophobic interaction chromatography media.

 

Hipersep®-Bio Liquid Handling Systems

 

Hipersep®-Bio	S	M	L	XL
Flow rate (L/h)	15-150	50-500	150-1500	300-3000

Novasep’s engineers can design customized units incorporating columns with up to 2000 mm internal diameter, ideally suited to your facilities and specific process needs. Hipersep®-Bio Liquid Handling Systems can accomodate a flexible number of buffer & CIP inlets and up to 10 fractions.

Materials of construction

• 316 L Stainless Steel, acrylic, glass: borosilicate type I, hastelloy, Teflon® seal (compatible with all organic solvents) or EPDM
• Full material certification package
• Safety & ergonomic tools
• Sanitary fittings, internal finish better than 0.4 μm, electropolished

Smart Fraction Collection System

• Based on time and/or detection threshold and/or slope
• Based on volume
• Based on time/volume after an event

Quality

• Complete IQ/OQ and PQ (PQ optional)
• DESP or ASME/BPE: code of construction

Software - Hipersep® SE-Bio

The Hipersep® SE-Bio software operates and monitors all LPLC and MPLC systems according to customers' needs:

• Isocratic, step or linear gradients
• In-line buffer dilution
• Design for bio-applications
• Validated software compliant with 21 CFR part 11 and GAMP 4
• User-friendly, designed for chromatographers and manufacturers

Options

• Temperature control
• Design for ATEX zone
• Tankfarms and solvent recovery systems
• Column packing units
• SIP

Benefits and Advantages

Experience

For more than 10 years, Novasep has been designing, building and selling optimized Prochrom® chromatography columns and systems for your biopharma purification applications.
Novasep provides product and process development as well as fully automated chromatographic systems that integrate easily and perfectly in your process.

Scalability

LPLC and MPLC systems incorporate Prochrom® chromatography columns with bed volumes from 10 to 400 L and column diameters from 200 mm to 2000 mm. We can develop custom-made design onrequest. We provide Prochrom® chromatography columns for your scale-up solution.

Expertise

Unique teams of chemists, biochemists, process and computer engineers combine their complementary talents to develop and continuously improve purification processes. 

Novasep Services

Novasep combines the complementary talents of chemical, electrical and automation engineers as well as chemists to offer its expertise in customized services suited to your expectations.