See our Chromatography technology page.
The most popular method for the purification of proteins and other charged molecules is ion exchange chromatography. In cation exchange chromatography, positively charged molecules are attracted to a negatively charged solid support. Conversely, in anion exchange chromatography, negatively charged molecules are attracted to a positively charged solid support. To optimize the binding of all charged molecules, the mobile phase is generally a low to medium conductivity (salt concentration) solution.
The adsorption of the molecules to the solid support is driven by the ionic interaction between the two moieties and binding capacities are generally quite high. The strength of the interaction is determined by the number and location of the charges on the molecule and solid support.
By increasing the salt concentration (generally a linear salt gradient), the molecules with the weakest ionic interactions are disrupted first and elute earlier in the salt gradient. Those molecules that have a very strong ionic interaction require a higher salt concentration and elute later in the gradient.
Typical example of ion exchange technology
Filtration processes using membranes and Cross-Flow Filtration (CFF) consist of separating dissolved or suspended elements, in relation to their molecular weight cut-off. When creating a pressure difference between retentat and permeate, the membrane acts as a filter.
The substances smaller than the membrane pores are combined with the solvent, named permeate, while the larger molecules or particles are retained as retentat.
For highly demanding or very large scale applications, we offer cross-flow filtration systems (CFF) from ultrafiltration to microfiltration including reliable mineral Kerasep® membranes.
Evaporation and crystallization equipment can be configured as multiple-effect, thermo-compression and mechanical recompression. They can be fully integrated in processes developed by Novasep.
Click here to discover our evaporation and crystallization system : Evaporation/Crystallization systems.
We combine the complementary talents of agrochemists and process engineers to offer our expertise in Process Development Services suited to your expectations.
Download our brochures for ingredient purification and production (bulk, food, functional).
For any questions on Novasep's purification/separation services and equipment for industrial biotech applications, please contact us.