Contract manufacturing services for recombinant proteins

Contract manufacturing services for recombinant proteins


Novasep is a trustable and agile partner to drive your project to success. Over the years we have manufactured a large number of recombinant proteins using various expression platforms.Novasep CMO Fermentor

Expert in cell-culture and fermentation processes, we can produce your:

  • Antigens from virus, bacteria or parasites
  • Fusion proteins and conjugated proteins
  • Enzymes and other metabolites (IFN, IL-10, etc.)


Unique capabilities and methodology

We offer the following range of services:

  • Full process and technological transfer
  • Upstream process development:
    • Cell adaptation to serum-free, suspension growth
    • Creation of Master Cell Bank (MCB) and Working Cell Bank (WCB)
    • Optimization of cell culture and fermentation parameters (feeding strategy…)
    • Conditions of expression induction optimization
    • Lysis conditions optimization
  • Downstream process development
    • Clarification optimization
    • Optimization of purification steps (filtration and chromatography operations)
    • Virus inactivation conditions
  • Process scale-up and validation
  • Aseptic F&F for liquids in glass vials
  • Full analytical transfer, development and qualification


Capacities and facilities

Novasep CMO Cell CultureOur two sites in Belgium (Seneffe and Gosselies) are specialized in the production of recombinant proteins.

Process development labs and offices: 3,000 m² 
Upstream and downstream cGMP suites: 900 m²
Dedicated area for hazardous biologics: 120m² 
QC labs: 300 m²

Upstream Processing

Cell Culture

Up to 1,000 L for cGMP production
Static, micro-carier, suspension
Batch, fed-batch, perfusion (ATF)


Up to 100 L for cGMP production
Static, suspension
Batch, fed-batch

expression systems

CHO, VERO, HEK, MRC-5, SP2/0, 3T3, MDCK, NS0, proprietary cell lines, etc.

Bacteria and yeasts

E.coli, Staphylococcus, Shigella, V.cholera, Lactococcus lactis,
Meningitidis B., BCG, L.lactis, P.pastoris, etc.

Insect cell lines

SF9, SF21, Hi5, etc.


Downstream Processing


From 10 to 300 mm I.D.
Packed or expanded bed


TFF units for MF and UF/DF
Virus removal filters


Our track record

We have a rich and extensive experience in the cGMP production and purification of multiple recombinant or native proteins including:

  • Large panel of viral proteins from HPV, VZV, CMV, EBV, HSV, etc.
  • Parasites proteins (for example from Plasmodium, responsible of malaria)
  • Lipo-polysccharides from V.cholerae, N.meningitidis, etc.
  • Conjugated proteins with polysaccharides and fusion proteins
  • Monoclonal antibodies and mAb fragments
  • Enzymes
  • Hormones/cytokines & growth factors including IFN, IL-10, hTFF1, etc.

Our BSL2/BSL3 pathogens manufacturing facility, dedicated to contained production, can be proposed to manufacture hazardous biologics or compounds such as toxins and allergens. Learn more about our BSL3 pathogens manufacturing capabilities.

  • Global offer for biopharmaceuticals
    Global offer for biopharmaceuticals
    size: 140.8K
    date: 08/12/2014
  • ADC Brochure
    ADC Brochure
    size: 672.1K
    date: 20/10/2015
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