Preparative Supercritical Fluid Chromatography (SFC) Technology from Novasep

The quickest preparative chromatography technique

Preparative SFC

Preparative SFC is quite similar to preparative HPLC. However, for preparative supercritical fluid chromatography, the eluent is a mixture of supercritical carbon dioxide and generally up to 20% (sometimes up to 40%) of organic co-solvent (usually an alcohol).

Thanks to the low viscosity and high diffusivity of supercritical CO₂, resolutions using SFC are 3 to 5 times faster than with HPLC. In addition, as the CO₂ evaporates, the isolation of the compound of interest is also very quick. In the lab, this technique uses 3 to 20 times less organic solvent than for HPLC. Moreover, in well engineered preparative SFC systems, the CO₂ is recycled, further improving the process economics and reducing its environmental impact, making it a green separation process in the lab and in the pilot plant. SFC is a very quick chromatography technique and, thus, ideal in early development to produce grams to multi-kg amounts of desired products.

Click here to discover our preparative SFC systems: Prochrom® Supersep.

Carbon dioxide recycling for cost-effective and greener separations

Except for analytical and small preparative instruments, CO₂ should be recycled, otherwise carbon dioxide consumption would easily exceed 10 or even 20 kg of liquefied gas per hour for a preparative SFC system equipped with a 50 mm I.D. column. Novasep’s systems integrate CO₂ recycling technology very effectively.

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