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Home / Equipment Solutions / HPLC skids and columns / Prochrom®, best-in-class preparative HPLC solutions

Prochrom®, best-in-class preparative HPLC solutions


Our Prochrom® technology is recognized as the reference in preparative and process-scale HPLC solutions for the purification of APIsHPAPIspeptides, oligonucleotides, recombinant proteins (including insulin), cannabinoids and other small molecules for the fine chemicals industry obtained by fermentation, extraction, semi-synthesis or organic synthesis.

Depending on molecule properties (size, charge, hydrophobicity, biorecognition), our product ranges allow for several purification techniques:

  • Reverse Phase Chromatography (RP HPLC),
  • Normal Phase Chromatography (NP HPLC),
  • Hydrophobic Interaction Chromatography (HIC),
  • Ion Exchange Chromatography (Anion and Cation) (IEX),
  • Size Exclusion Chromatography (SEC),
  • Affinity Chromatography (AC).

Prochrom® Columns, HPLC column for pharmaceutical applications

Novasep has decades of experience in developing, using and supplying preparative HPLC columns and skids for intermediate purification and polishing of APIs, biologics, and other small molecules. Our Prochrom® range offers state-of-the-art industrial HPLC, from the development phase to large-scale commercial production.

Prochrom HPLC Chromatography Column

Dynamic Axial Compression (DAC)

Some of our columns have been running for decades without unpacking and without any loss of performance. This performance consistency over time is due to Dynamic Axial Compression (DAC) technology.

Pioneered by Novasep, this technology is based on the use of a hydraulically actuated piston offering the highest efficiency and peak symmetry. Combined with our proprietary flow distributors, the DAC technology ensures optimum performance for an extended period of time from 50 to 1,2000 mm I.D. columns. We guarantee the performance of your scaled-up process using our Prochrom® columns.


Prochrom® range is well-known for its longevity and reliability. Columns installed in production plants in the 1980s are still in operation today. We guarantee the performance and robustness of both our systems and processes.

Prochrom HPLC Chromatography Column Robustness Prochrom HPLC Chromatography Column Maintenance

A complete toolbox

Our columns are designed to facilitate cleaning and maintenance operations while maximizing operator safety: all heavy parts are manipulated from the bottom of the column, thus avoiding hoists and overhead manipulation; handling tools such as a piston rotation unit and piston/flange tables as accessories. Specially designed to be user-friendly and easy-to-use, one single operator can perform all of these operations.

A solid experience and track record

  • More than 30 years of experience in column design and manufacturing.
  • More than 150 engineers and technicians continuously improving the reliability, ergonomics and performance of our systems.
  • More than 2,500 columns purifying peptides, APIs and intermediates every day, all around the world.

A complete range from Pilot to industrial scale

Prochrom® columns LC50 LC80 LC110 LC150 LC200 LC300 LC450 LC590 LC800 LC1000 LC1200 Above
Max. bed height (mm) 300 350 400  Upon request  
Pressure rating (bar) 100 70
Compression features Dynamic Axial Compression (DAC)
Pressure transmitter N/A Included
Temperature jacket Yes Optional
Material wetted parts Stainless Steel (316 & 316L), PTFE and FEP  
Columns I.D. (mm) 50 80 110 150 200 300 450 590 800 1000 1200  


Hipersep®, Best-in-class HPLC chromatography systems for pharmaceutical applications

Hipersep® systems are automated high-pressure solvent delivery modules for preparative chromatography under cGMP conditions.

They have been engineered to guarantee the best chromatographic efficiency in combination with Prochrom® columns through an optimized layout ensuring lowest dead volumes and turbulent flow regimes.

Hipersep® Pilot, our new modular, compact and ergonomic system

Hipersep Pilot Hplc Chromatography System skid

Hipersep® Pilot is designed to minimize sanitary cross-contamination risk and which, with over 1,000 possible configurations, will integrate with any process.

Cyclojet is an innovative chromatography process designed for optimal productivity, particularly in cases of limited batch process resolution.


Explore Hipersep® Pilotdownload cyclojet

 Process M Website Banner

Process Analytical Technology

Our equipment includes advanced PAT (Process Analytical Technology guidelines of the FDA) compliant features such as a high performance gradient module as well as smart fraction collection software.

Software and automation

Hipersep Software HPLC Chromatography System

The Hipersep® SC software operates and monitors all HPLC systems according to our customers’ needs.

The monitoring software remains the same on the whole Hipersep® range, easing scale-up.

  • Reliable, validated and with guaranteed traceability
  • Complete quality documentation
  • Accurate control of all critical process parameters
  • Programmable Logic Controller (PLC) with PC user interface
  • Multiple access levels
  • User-friendly
  • Supplier software validation performed
  • Software developed to be compliant with 21 CFR part 11 and GAMP5 Guidelines

A complete range of systems from Pilot to industrial production

Hipersep®PilotProcess MLXLXXL
Productivity range (kg/day) in Batch mode 0.2-4 2-33 17-57 29-106 53-165
Productivity range (kg/day) in Cyclojet/Varicol® 8-12 14-99 114-171 212-318 330-495
Typical Prochrom® column diameters (mm) 50 / 80 / 110 / 150 110 / 150 / 200 / 300 / 450 450 / 590 590 / 800 800 / 1000
Skid flowrate (L/h) 6*-90 20 to 200 low flow version
60 to 500 normal flow version
300-1000 500–1600 1000-2500
Gradient accuracy (%) < 1
Max pressure (bar) 100 70
Explosion-proof ATEX zone II, Class 1 Divison 2
Certifications / Equipment standards CODAP, ASME for columns CE and UL for systems
Configurable options High pressure injection module, Eluent & feed filters, Heat exchanger,Backflush configuration valves, pH conductivity, Nitrogen flush for product recovery, Up to 10 collections, Solvent recycling, Cyclojet process

 *Gradient accuracy <2% below 10L/h

 In addition to standard features, our Hipersep® can include options such as:

  • 5 to 10 additional collection valves
  • Recirculation valves to reduce solvent consumption
  • A heat-exchanger in order to control solvent temperature adapted to temperature-sensitive purification and locations with difficult temperature conditions
  • A column back-flush valve enabling elution direction to be reversed into the column

Packing in less than 5 minutes: Pack-n-Sep

Novasep Prochrom HPLC Pack N Sep

For convenient and fast packing, we have also developed Prochrom® Pack-n-Sep lab columns. This line of equipment combines the advantages of both Prochrom® DAC columns and an ultra-fast packer. This technology is designed for our customers maintaining a range of pre-packed columns, or in combination with our Varicol® continuous chromatography equipment.

This packer enables packing or unpacking in less than 5 minutes.

You only need one packer for several 50 or 80 mm I.D. columns.  Columns can be used on the packer in DAC mode or detached, a proprietary compression device maintaining bed compression.


Related Documents


Brochure Icon Insulin Purification Download
Brochure Icon Industrial Cannabinoid Purification Download
Brochure Icon Peptides and Oligonucleotides purification Download

Application note

Brochure Icon How does Hipersep® Pilot, with Cyclojet process, answer new requirements in the pharma industry? Download
Brochure Icon Successful Cannabinoid Purification and Scale-Up Download


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