Molecules reaching the market in the recent years present an ever-growing complexity. In the meantime, purity criteria are more important than ever to ensure patients safety. The combination of these two facts represents a big challenge for the manufacturing industry where the right combination of state-of-the-art synthesis and advanced purification is necessary to meet the demand in a cost-effective and time-efficient manner. This paper presents membrane filtration and chromatography as efficient technologies to reach the required purity level. The use of these technologies at industrial scale is illustrated through a case study including a complex biocatalysis reaction and the complete downstream processing yielding the final, pure product. The scale-up from gram to hundred kilogram scale, process efficiency and timelines are also considered.