Prochrom® Columns and Systems are designed for preparative and process-scale HPLC chromatography

Our Prochrom^® technology is recognized as the reference in preparative and process-scale HPLC solutions for the purification of APIs, HPAPIs, peptides, oligonucleotides, recombinant proteins (including insulin), cannabinoids and other small molecules for the fine chemicals industry obtained by fermentation, extraction, semi-synthesis or organic synthesis.

As a complete HPLC solution, the Prochrom^® range includes:

Prochrom^®Columns, HPLC column for pharmaceutical applications

Novasep has decades of experience in developing, using and supplying preparative HPLC systems for intermediate purification and polishing of APIs, biologics, and other small molecules. Our Prochrom^® range offers state-of-the-art industrial HPLC, from the development phase to large-scale commercial production.

Dynamic Axial Compression (DAC)

Some of our columns have been running for decades without unpacking and without any loss of performance. This performance consistency over time is due to Dynamic Axial Compression (DAC) technology.
Pioneered by Novasep, this technology is based on the use of a hydraulically actuated piston offering the highest efficiency and peak symmetry. Combined with our proprietary flow distributors, the DAC technology ensures optimum performance for an extended period of time from 50 to 1,2000 mm I.D. columns.
We guarantee the performance of your scaled-up process using our Prochrom^® columns.

Robustness

Prochrom^® columns are known for their longevity and reliability. Prochrom^® columns installed in production plants in the 1980s are still in operation today. We guarantee the performance and robustness of both our systems and processes.

A complete toolbox

Our columns are designed to facilitate cleaning and maintenance operations while maximizing operator safety: all heavy parts are manipulated from the bottom of the column, thus avoiding hoists and overhead manipulation; handling tools such as a piston rotation unit and piston/flange tables as accessories. Specially designed to be user-friendly and easy-to-use, one single operator can perform all of these operations.

A solid experience and track record

More than 30 years’ experience in column design and manufacturing.
More than 150 engineers and technicians continuously improving the reliability, ergonomics and performance of our systems.
More than 2,500 columns purifying peptides, APIs and intermediates every day, all around the world.

A complete range from Pilot to industrial scale

Prochrom^®columns	LC50	LC80	LC110	LC150	LC200	LC300	LC450	LC590	LC800	LC1000	LC1200	Above
Max. bed length (mm)	300	350	400
Pressure rating (bar)	100							70 - 100			70	Upon request
Compression features	Dynamic Axial Compression (DAC)
Pressure transmitter	N/A				Optional
Temperature jacket	Yes		Optional
Material wetted parts	Stainless Steel (316 & 316L), PTFE and FEP
Columns I.D. (mm)	50	80	110	150	200	300	450	590	800	1000	1200

Hipersep^®, Best-in-class HPLC chromatography skids for pharmaceutical applications

Hipersep^® systems are automated high-pressure solvent delivery modules for preparative chromatography under cGMP conditions.

They have been engineered to guarantee the best chromatographic efficiency in combination with Prochrom^® columns through an optimized layout ensuring lowest dead volumes and turbulent flow regimes.

Hipersep^®Pilot, our new modular, compact and ergonomic system

Hipersep® Pilot is our new modular, compact and ergonomic system which is designed to minimize sanitary cross-contamination risk and which, with over 1,000 possible configurations, will integrate with any process.

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Cyclojet is an innovative chromatography process designed for optimal productivity, particularly in cases of limited batch process resolution.

Download the Cyclojet application note >

Process Analytical Technology

Our equipment includes advanced PAT (Process Analytical Technology guidelines of the FDA) compliant features such as a high performance gradient module as well as smart fraction collection software.

Software and automation

The Hipersep^® SC software operates and monitors all HPLC systems according to our customers’ needs.

The monitoring software remains the same on the whole Hipersep® range, easing scale-up.

Reliable, validated and with guaranteed traceability
Complete quality documentation
Accurate control of all critical process parameters
Programmable Logic Controller (PLC) with PC user interface
Multiple access levels
User-friendly
Supplier software validation performed
Software developed to be compliant with 21 CFR part 11 and GAMP5 Guidelines

A complete range of systems from Pilot to industrial production

Hipersep^®	Pilot	XS	S	M	L	XL	XXL
Productivity range (kg/day) in Batch mode	0.2-4	2-7	2-7	7-33	17-57	29-106	53-165
Productivity range (kg/day) in Cyclojet/Varicol®	8-12	14-21	14-21	66-99	114-171	212-318	330-495
Typical Prochrom® column diameters (mm)	50 80110150	150200	150200	300450	450590	590800	8001000
Skid flowrate (L/h)	6*-90	60-200	90-200	150-500	300-1000	500–1600	1000-2500
Gradient accuracy (%)	< 1
Max pressure (bar)	100						70
Explosion-proof	ATEX zone IIClass 1 Divison 2
Certifications / Equipment standards	CODAP/ASME for columns CE/UL for systems
Configurable options	High pressure injection module, Eluent & feed filters, Heat exchanger,Backflush configuration valves, pH conductivity, Nitrogen flush for product recovery, Up to 10 collections, Solvent recycling, Cyclojet process

*Gradient accuracy <2% below 10L/h

In addition to standard features, our Hipersep^® can include options such as:

5 to 10 additional collection valves
Recirculation valves to reduce solvent consumption
A heat-exchanger in order to control solvent temperature adapted to temperature-sensitive purification and locations with difficult temperature conditions
A column back-flush valve enabling elution direction to be reversed into the column

Solvent recycling: reduce solvent consumption by up to 99%

Upon request, we can combine our chromatography equipment with automated solvent recycling technology. This enables the recovery of up to 99% of the solvent in both isocratic and gradient conditions, thus dramatically reducing your operating costs and environmental footprint. It enables the recovery of up to 99% of the solvent for both isocratic and gradient conditions, thus dramatically reducing your operating costs and environment footprint.

During a separation, the solvent is recovered from the evaporators (and optionally dryers) while purified, concentrated or dry compounds are recovered. The recycled solvent is used to elute the column and to dissolve the dry feed mixture. Only a small amount of fresh solvent is automatically added to the recycled eluent stream in order to automatically adjust the eluent composition using a proprietary technology.

Packing in less than 5 minutes: Pack-n-Sep

For convenient and fast packing, we have also developed Prochrom^® Pack-n-Sep lab columns. This line of equipment combines the advantages of both Prochrom^® DAC columns and an ultra-fast packer. This technology is designed for our customers maintaining a range of pre-packed columns, or in combination with our Varicol^® continuous chromatography equipment.

This packer enables packing or unpacking in less than 5 minutes.

You only need one packer for several 50 or 80 mm I.D. columns. Columns can be used on the packer in DAC mode or detached, a proprietary compression device maintaining bed compression.